RESUMO
In Navarre, Spain, in May 2022, the seroprevalence of anti-nucleocapsid (N) and anti-spike (S) antibodies of SARS-CoV-2 was 58.9% and 92.7%, respectively. The incidence of confirmed COVID-19 thereafter through July was lower in people with anti-N antibodies (adjusted odds ratio (aOR)â¯=â¯0.08; 95% confidence interval (CI): 0.05-0.13) but not with anti-S antibodies (aORâ¯=â¯1.06; 95% CI: 0.47-2.38). Hybrid immunity, including anti-N antibodies induced by natural exposure to SARS-CoV-2, seems essential in preventing Omicron COVID-19 cases.
Assuntos
Anticorpos Antivirais , COVID-19 , Anticorpos Antivirais/sangue , COVID-19/epidemiologia , COVID-19/imunologia , Humanos , Proteínas do Nucleocapsídeo , SARS-CoV-2 , Estudos Soroepidemiológicos , Espanha/epidemiologia , Glicoproteína da Espícula de CoronavírusRESUMO
Circulating recombinant forms (CRFs) contribute substantially to the HIV-1 pandemic. Among 105 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here we find that it belongs to a larger cluster incorporating 39 viruses collected in 7 countries from 3 continents, 22 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). This BF cluster comprises three major subclusters, two associated with Bolivian and one with Peruvian individuals. Near full-length genome sequence analyses of nine viruses, collected in Spain, Bolivia, and Peru, revealed coincident BF mosaic structures, with 13 breakpoints, 6 and 7 of which coincided with CRF12_BF and CRF17_BF, respectively. In a phylogenetic tree, they grouped in a clade closely related to these CRFs, and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allowed to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1986. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF.
Assuntos
Variação Genética , Genoma Viral , Infecções por HIV/genética , HIV-1/genética , Filogenia , Recombinação Genética , Adulto , Feminino , Infecções por HIV/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Análise de Sequência de DNA , América do Sul/epidemiologiaAssuntos
Infecções Comunitárias Adquiridas , Infecções por HIV , Neoplasias Pulmonares , Pneumonia , Infecções Comunitárias Adquiridas/diagnóstico , Infecções Comunitárias Adquiridas/epidemiologia , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Teste de HIV , Hospitais de Ensino , Humanos , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/epidemiologia , Espanha/epidemiologiaRESUMO
No disponible
Assuntos
Humanos , Feminino , Idoso , Infecções por Corynebacterium/etiologia , Infecções Respiratórias/complicações , Complicações do Diabetes , Corynebacterium diphtheriae/isolamento & purificação , Infecções por Corynebacterium/diagnóstico , Infecções por Corynebacterium/microbiologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Antibacterianos/uso terapêutico , Eritromicina/uso terapêuticoRESUMO
El uso de los dispositivos semicríticos reutilizables se ha extendido en la práctica médica actual tanto con fines diagnósticos como terapéuticos. Sin embargo, la reutilización de estos instrumentos conlleva el riesgo de una transmisión cruzada de microorganismos de un paciente a otro. El proceso de limpieza y desinfección de estos dispositivos es complejo, largo, caro y muy sensible a que se produzcan fallos. En el presente documento se analizan los aspectos epidemiológicos de las infecciones asociadas a la reutilización de los dispositivos semicríticos, y el papel del laboratorio de Microbiología en la monitorización del proceso de limpieza y desinfección de los mismos a través de los controles microbiológicos. Se revisan las recomendaciones de diferentes sociedades científicas sobre la pertinencia de dichos controles y se establecen recomendaciones específicas para la toma y el procesamiento de las muestras, la interpretación de los resultados y las medidas a tomar en función de los resultados obtenidos
The use of reusable semi-critical devices has been extended in current medical practice for both diagnostic and therapeutic purposes. However, reuse of these instruments carries the risk of cross-transmission of microorganisms from one patient to another. The process of cleaning and disinfecting these devices is complex, long, expensive and very error-prone. This paper analyses the epidemiological aspects of infections associated with the reuse of semi-critical devices and the role of the Microbiology laboratory in monitoring the cleaning and disinfecting process through microbiological controls. The recommendations of different scientific societies on the relevance of such controls are reviewed and specific recommendations are proposed for the taking and processing of the samples, interpretation of the results and measures to be taken depending on the results obtained
Assuntos
Humanos , Monitoramento Ambiental/métodos , Desinfecção/métodos , Esterilização/métodos , Equipamentos e Provisões/microbiologiaAssuntos
Infecções por Escherichia coli/transmissão , Escherichia coli/efeitos dos fármacos , beta-Lactamases/genética , Idoso , Idoso de 80 Anos ou mais , Técnicas de Tipagem Bacteriana , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Farmacorresistência Bacteriana Múltipla/genética , Escherichia coli/genética , Escherichia coli/patogenicidade , Infecções por Escherichia coli/microbiologia , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Tipagem de Sequências MultilocusRESUMO
The use of reusable semi-critical devices has been extended in current medical practice for both diagnostic and therapeutic purposes. However, reuse of these instruments carries the risk of cross-transmission of microorganisms from one patient to another. The process of cleaning and disinfecting these devices is complex, long, expensive and very error-prone. This paper analyses the epidemiological aspects of infections associated with the reuse of semi-critical devices and the role of the Microbiology laboratory in monitoring the cleaning and disinfecting process through microbiological controls. The recommendations of different scientific societies on the relevance of such controls are reviewed and specific recommendations are proposed for the taking and processing of the samples, interpretation of the results and measures to be taken depending on the results obtained.
Assuntos
Infecção Hospitalar/prevenção & controle , Contaminação de Equipamentos/prevenção & controle , Esterilização , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Desinfecção/métodos , Desinfecção/normas , Reutilização de Equipamento , Humanos , Técnicas Microbiológicas , Guias de Prática Clínica como Assunto , Esterilização/métodos , Esterilização/normasRESUMO
The environmental microbiological control is necessary to prevent infections associated with certain procedures that are performed at the hospital. In this review the procedures for control of water and dialysis fluids, and air in operating rooms and immunocompromised units are addressed. The dialysis quality management guidelines define the highest levels of chemical, microbiological and endotoxin in purified water and dialysis fluids based on the recommendations of scientific societies. The microbiological control of water and dialysis fluids should include detection of microorganisms and endotoxin levels. Regarding the microbiological air sampling of operating rooms and immunocompromised units the types of clean rooms in which is recommended to perform microbiological air monitoring; the sample collection methods; culture media; incubation conditions; the most common microorganisms, and permissible levels depending on the type of surgery are described.
Assuntos
Controle de Infecções/métodos , Salas Cirúrgicas/normas , Diálise Renal/normas , Microbiologia do Ar/normas , Líquidos Corporais/microbiologia , Meios de Cultura , Ambiente Controlado , Monitoramento Ambiental , Humanos , Microbiologia da ÁguaRESUMO
El control microbiológico ambiental es necesario para evitar infecciones relacionadas con determinados procedimientos que se llevan a cabo en el hospital. En esta revisión se abordan los procedimientos de control del agua y líquidos en las unidades de diálisis y de aire en quirófano y unidades de inmunodeprimidos. Las guías de gestión de calidad del líquido de diálisis definen los niveles máximos de contaminación química, microbiológica y de endotoxinas tanto del agua purificada como del líquido de diálisis. El control microbiológico del agua y del líquido de diálisis debe incluir la detección de microorganismos y de los niveles de endotoxinas. Respecto al control microbiológico del aire de los quirófanos y unidades de inmunodeprimidos, en esta revisión se definen los tipos de salas limpias en los que hay que realizar controles microbiológicos del aire; los métodos de recogida de muestras; los medios de cultivo y las condiciones de incubación; los microorganismos más frecuentes, y los niveles admisibles según el tipo de quirófano
The environmental microbiological control is necessary to prevent infections associated with certain procedures that are performed at the hospital. In this review the procedures for control of water and dialysis fluids, and air in operating rooms and immunocompromised units are addressed. The dialysis quality management guidelines define the highest levels of chemical, microbiological and endotoxin in purified water and dialysis fluids based on the recommendations of scientific societies. The microbiological control of water and dialysis fluids should include detection of microorganisms and endotoxin levels. Regarding the microbiological air sampling of operating rooms and immunocompromised units the types of clean rooms in which is recommended to perform microbiological air monitoring; the sample collection methods; culture media; incubation conditions; the most common microorganisms, and permissible levels depending on the type of surgery are described
Assuntos
Humanos , Diálise Renal/normas , Controle de Infecções/métodos , Salas Cirúrgicas/normas , Microbiologia da Água , Microbiologia do Ar/normas , Líquidos Corporais/microbiologia , Monitoramento Ambiental , Meios de CulturaRESUMO
BACKGROUND: Salmonella gastro-enteritis is a Zoonoses transmitted by the ingestion of food products and water or fomites contaminated by the faeces of infected people or animals. At present, constitutes a world-wide pandemic. The aim of the present study has been to in progress examine cases of non-typhoidal salmonellosis in the Health Area I of Navarra (376,079 inhabitants). METHODS: 39,697 outpatient specimens submitted for culture during 1993-2000 were analysed retrospectively. Standard procedures to isolate enteropathogens were employed. The Salmonella strains were serotyped. Data was collected on age, sex, specimen date and result of culture and antimicrobial susceptibility testing for all isolates. RESULTS: 2,924 salmonellae were isolated (7.4%) with the most frequent serotype being Salmonella Enteritidis (62%). The highest isolation rate was associated with children, particularly infants (1,117.3 per 100,000 inhabitants). Salmonella Typhimurium was typically more resistant than Salmonella Enteritidis, although resistance rates in both have increased in recent time. CONCLUSIONS: In spite of the socio-economic improvements, the incidence of gastro-enteritis associated with Salmonella spp. has continued to increase in recent years, mainly affecting infants, and constitutes an important public health problem.
Assuntos
Infecções por Salmonella/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Pré-Escolar , Gastroenterite/epidemiologia , Gastroenterite/etiologia , Humanos , Lactente , Pessoa de Meia-Idade , Estudos Retrospectivos , Intoxicação Alimentar por Salmonella/epidemiologia , Infecções por Salmonella/microbiologia , Salmonella enteritidis/isolamento & purificação , Salmonella typhimurium/isolamento & purificação , Espanha/epidemiologiaRESUMO
Fundamento: La gastroenteritis por Salmonella es una zoonosis que se transmite por la ingestión de alimentos, agua o fomites contaminados por las heces de un animal o persona infectados y constituye una pandemia de distribución mundial. El objetivo del trabajo ha sido el estudio de la evolución de la salmonelosis no tifoidea en el Área de Salud I de Navarra (376.079 habitantes).Método: Se analizaron retrospectivamente 39.697 coprocultivos extrahospitalarios realizados durante 1993-2000. Para el aislamiento de enteropatógenos bacterianos se emplearon métodos convencionales. Las cepas de Salmonella spp fueron serotipificadas. Otras variables estudiadas fueron edad, sexo, fecha de aislamiento y sensibilidad a antimicrobianos. Resultados: Se aislaron 2.924 Salmonella spp (7,4 per cent). El serotipo más frecuente fue Salmonella Enteritidis (62 per cent). La tasa de mayor incidencia se dio en la edad pediátrica y especialmente en los menores de 1 año (1.117,3 ´ 100.000 habitantes).El serotipo Typhimurium fue globalmente más resistente que el serotipo Enteritidis, aunque en ambos se ha constatado con el tiempo un aumento de las resistencias. Conclusiones: a pesar de las mejoras socioeconómicas y de la calidad de vida, la gastroenteritis por Salmonella spp ha ido en aumento en los últimos años, afectando sobre todo a los niños más pequeños y constituyendo un importante problema de salud pública (AU)